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The Journal of Immunology, 1963, 91: 851-858.
Copyright © 1963 by The American Association of Immunologists, Inc.

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Chromatographic Separation of the First Component of Complement and Its Assay on a Molecular Basis

Tibor Borsos1 and Herbert J. Rapp

From the Immunology Section, Diagnostic Research Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Abstract

A rapid and simple procedure for separating the first component (C'1a) of guinea pig complement in stable form is described as well as a quantitatative method for measuring C'1a activity on a molecular basis.

Footnotes

1 This work was begun at the Department of Microbiology, Johns Hopkins University School of Medicine during the tenure of a Career Development Fellowship, United States Public Health Service; it was supported by ONR Contract # Nonr 248(60), NSF Grant #G19372, and NIH Grant #AI 2566.




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