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The Temporal Synthesis of Chicken Antibodies

The Effect of Salt on the Precipitin Reaction¹

From the Department of Bacteriology and the Department of Comparative Biochemistry and Physiology, University of Kansas, Lawrence, Kansas

Abstract

Evidence was presented that primary chicken anti-bovine serum albumin (BSA) antisera contain a high molecular weight antibody which precipitates in 0.15 M NaCl and which agglutinates sensitized red cells, a 7 S non-hemagglutinating antibody which precipitates only in 1.5 M NaCl when isolated from the heavier fraction by zone ultracentrifugation, and a 7 S hemagglutinating antibody which precipitates in 0.15 M NaCl and which is also found in high concentrations in hyperimmune sera.

Footnotes

¹ This investigation was supported in part by Public Health Service Training Grant 2E-158 from the National Institute of Allergy and Infectious Diseases, Public Health Service.

Presented in part at the annual meeting of the American Association of Immunologists, Atlantic City, New Jersey, April 1963.

² Present address: Department of Microbiology, University of Hawaii, Honolulu, Hawaii.

³ United States Public Health Service Senior Fellowship Grant GM-K3-5992.