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The Journal of Immunology, 1963, 91: 683-692.
Copyright © 1963 by The American Association of Immunologists, Inc.
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Antigenic Components of Human Erythrocytes1

Calderon Howe, Stratis Avrameas, Christiane de Vaux St. Cyr, Pierre Grabar and Lucille T. Lee

From the Institute for Cancer Research, Villejuif, Seine, France, and the Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York

Abstract

The stroma of erythrocytes obtained from individual donors was analyzed by electrophoresis, and by immunoelectrophoresis with concentrated rabbit antisera to whole hemolysate and post-hemolytic residue. Each antiserum gave identical reaction patterns with all preparations of stroma irrespective of blood groups. At least 12 antigenic constituents besides hemoglobin were demonstrated, five of which were recognized as enzymes. In addition, four isoenzymes with lactic dehydrogenase activity were encountered. Purified virus receptor substance, glycoprotein complex obtained by butanol treatment of stromal residues, and glycoprotein in aqueous extracts of stroma exhibited basic immunologic identity unrelated to blood groups. Besides M or N activity corresponding to the donor's MN group, traces of blood group A activity were demonstrable in virus receptor substance and in butanol aqueous fractions prepared from cells of group A1. The relation of these various components to the erythrocyte membrane ultrastructure is considered.

Footnotes

1 This investigation was supported in part by Research Grant AI-03168 and The General Research Support Grant from the National Institutes of Health, United States Public Health Service and The Commonwealth Fund.






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