HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Eisler, D. M. Articles by Metz, E. V. Search for Related Content PubMed PubMed Citation Articles by Eisler, D. M. Articles by Metz, E. V.

Anti-Pasteurella Pestis Factor in the Organs of Normal Mice and Guinea Pigs

I. Biologic Characteristics¹

From the Naval Biological Laboratory, School of Public Health, University of California, Berkeley, California

Abstract

The preparation from several organs of normal mice and guinea pigs of an anti-Pasteurella pestis factor (APF), active in vitro, is described. The APF was active in varying degrees for several strains of P. pestis and for Micrococcus lysodeikticus but not for several other Gram positive or negative bacteria.

Crude and purified APF from mouse liver and crude APF from mouse and guinea pig spleen and kidney were active following storage at -20°C. On the other hand, the activity of both crude and purified guinea pig liver APF was lost during storage at -20°C, but could be restored by heating at 60 or 65°C but not higher. Evidence is presented for the nonidentity of APF with previously described antibacterial substances such as basic polypeptides, histone, spermine, phagocytin and lysozyme. The evidence consists chiefly of dissimilarity in method of preparation and of the differential action of extraneous materials on the activity.

One dose of APF administered at the time of inoculation with P. pestis did not protect mice from infection, and the virulence of P. pestis for mice was not altered by preliminary treatment of the organisms with APF in vitro.

Footnotes

¹ This work was sponsored by the Office of Naval Research and the Bureau of Medicine and Surgery, U. S. Navy, under the terms of a contract between the Office of Naval Research and the Regents of the University of California. Reproduction in whole or in part is permitted for any purposes of the United States Government.