HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Merritt, K. Articles by Johnson, A. G. Search for Related Content PubMed PubMed Citation Articles by Merritt, K. Articles by Johnson, A. G.

Studies on the Adjuvant Action of Bacterial Endotoxins on Antibody Formation

V. The Influence of Endotoxin and 5-Fluoro-2-deoxyuridine on the Primary Antibody Response of the Balb Mouse to a Purified Protein Antigen¹

From the Department of Bacteriology, The University of Michigan, Ann Arbor, Michigan

Abstract

The adjuvant action of endotoxin on antibody formation to a single injection of a soluble protein antigen (BGG) was demonstrated in an inbred strain of Balb mice. The adjuvant action of endotoxin was expressed in this system by decreasing the induction period and increasing peak titer. The length of time in relation to antigen administration during which endotoxin would exert its adjuvant action was found to be longer in the mouse (from 7 days before to 6 days after antigen) than in previous studies in the rabbit.

The DNA analogue 5-fluoro-2'-deoxyuridine (FUDR) was found to inhibit antibody formation to BGG in these Balb mice when given daily in small doses or in a single large dose 24 or 48 hr after antigen. This single large dose of FUDR was found to be stimulatory to antibody formation when given near the time of antigen injection (1 hr before, with, or 1 hr after) or when given 4 days after antigen injection. No drug-induced tolerance to BGG in adult mice was achieved.

Footnotes

¹ This investigation was supported by Grant E 1524 from the USPHS and the Upjohn Co. The senior author received support from USPHS Training Grant 2G-500. Presented in part before the American Society for Microbiology, May 1962.