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The Presence of the Ragweed-Binding Antibodies in the _2A-, _2M- and -Globulins of the Sensitive Individuals¹

From the Department of Biochemistry Research, Roswell Park Memorial Institute, New York State Department of Health, and the Allergy Research Laboratory, Buffalo General Hospital, Buffalo 3, New York

Abstract

1. Sera from ragweed-sensitive individuals (two untreated and six treated) were examined by radioimmunoelectrophoresis to detect and identify the serum components possessing the binding activity for a purified fraction of ragweed (ragweed-binding antibodies). The method utilizes the fixation of I¹³¹-labeled ragweed antigen on the arcs formed by electrophoresed human antibody components and rabbit anti-human serum.

2. The ragweed-binding activity was detected in the -globulin of all the sera and in the _2A-globulin of all but one serum. Two sera showed the activity also in the _2M-globulin. In fact, all the sera contained the activity in the _2A- or in the _2M-globulin in addition to the activity in the -globulin. (One serum contained the activity in all three globulins, , _2A and _2M.) Such a high incidence of the _2A or _2M-antibody in sera of sensitive individuals suggests the association of the reaginic activity with these antibodies.

3. The ragweed-precipitating antibodies were detected in the ₂-globulin region of some sera including one from an untreated individual. The precipitating antibodies, when present in large amounts, interfered with the identification of antibody components. A modified technique, applicable to such precipitating systems, was described.

Footnotes

¹ Supported in part by Grant H 2092, from the National Heart Institute and Grant E 1303 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service.

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