HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Karabatsos, N. Search for Related Content PubMed PubMed Citation Articles by Karabatsos, N.

Hemolytic Properties of Eastern and Western Equine Encephalomyelitis Viruses¹

From the Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut

Abstract

Genetron-treated fluids of chick embryo cultures infected with Eastern and Western equine encephalomyelitis viruses were found, after freezing and thawing, to possess hemolytic activity over a wide temperature range (4 to 37°C). The virus hemolysin and hemagglutinin were inactivated by heat, whereas treatment with trypsin, ether and Ca⁺⁺ tended to cause a selective suppression of the hemolysin activity of both viruses. Certain changes in the pH at which assays were performed resulted in a reduction of hemolytic activity which was greater than any change in hemagglutinating activity. Treatment of chick red blood cells with RDE and trypsin failed to remove the receptors for either of the activities. However, trypsin treatment significantly increased the hemagglutinating activity of both viruses. The hemolysins were inhibited by specific viral antibody and were sedimentable with the hemagglutinins.

Footnotes

¹ This study was conducted under the auspices of the Commission on Viral Infections of the Armed Forces Epidemiological Board and was supported in part by the Office of the Surgeon General, Department of the Army, and in part by a grant (04566-O1A1) from the United States Public Health Service.