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Studies of the Immune Globulins of Human Serum

I. A Method for the Simultaneous Isolation of the Three Immune Globulins (_ss, _1M and _1A) from Individual Small Serum Samples¹

From the Department of Internal Medicine A Cliniques Universitaires St. Pierre, Louvain, Belgium, and the Institute of Bacteriology, University of Louvain, Belgium

Abstract

A method is described which allows the isolation of antigenically pure samples of the three immune globulins: _ss (=7 S ), _1M (=19 S ) and _1A (=_2A), starting from individual serum samples.

The _ss-globulin is obtained by chromatography on diethylaminoethyl-cellulose. Preparative ultracentrifugation in a sucrose density gradient is used to obtain the _1M-globulin from a euglobulin precipitate. The _1A-globulin is isolated from the remaining pseudoglobulin solution, by means of a modified fractionation procedure using precipitation with zine sulfate, preparative electrophoresis and salting out with ammonium sulfate, in the order indicated.

The purity and recovery rates of the three immune globulin fractions are assessed, and their value as representatives of the corresponding immune globulin groups of native serum is critically discussed.

Footnotes

¹ This investigation was supported by Public Health Service Grant 4263 (C1) from the United States Department of Health, Education and Welfare, and by Grant 270 from the Fonds de la Recherche Scientifique Médicale, Brussels, Belgium.

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