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The Use of Techniques of Absorption and Elution of Bovine Isoantibodies in the Study of Specificity of Antigen and Antibody and in the Development of Typing Reagents¹

From the Department of Dairy Science, Ohio State University, Columbus, Ohio

Abstract

Some modifications of a heat elution technique are described. Heat elution was used for recovery of cattle erythrocyte antibodies absorbed from isoimmune cattle sera. The results of the use of eluates as typing reagents and for studying serologic relationships between antibodies and between antigenic factors are listed and discussed. The use of elution techniques in studying specific properties of biologic materials is suggested.

Footnotes

¹ A cooperative project between the Dairy Cattle Research Branch of the ARS, USDA, the Department of Dairy Science of the Ohio State University, and the Ohio Agricultural Experiment Station, Columbus and Wooster, Ohio.

Part of the material presented in this article is taken from the Thesis of the senior author submitted for the degree of Master of Science at Ohio State University.