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The Journal of Immunology, 1963, 90: 960-970.
Copyright © 1963 by The American Association of Immunologists, Inc.
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Biologic Activities of Aggregated {gamma}-Globulin

VI. Aggregated Products of Rabbit Serum Proteins1

Takao Sugahara2, Kimishige Ishizaka2 and Teruko Ishizaka2

From the National Institute of Health, Tokyo, Japan

Abstract

Rabbit {alpha}-globulin, beta1-globulin and subfractions of {gamma}-globulin were aggregated by coupling with bis-diazotized benzidine and the aggregated products were studies for skin reactivity in normal guinea pigs and for C'-fixing properties. Induction of these biological activities upon nonspecific aggregation was characteristic of {gamma}-globulin only. Aggregated products of neither {alpha}-globulin nor beta1-globulin had either activity. Evidence was presented that only {gamma}-globulin had affinity for guinea pig tissues to which antibody combines upon passive sensitization. All three of these properties, namely skin reactivity and C'-fixing activity upon aggregation, and affinity for guinea pig tissue were present approximately equally in all the chromatographic subfractions of {gamma}-globulin.

All {gamma}-globulin subfractions of rabbit anti-ovalbumin sera contained antibody. Soluble antigen-antibody complexes prepared with these fractions gave skin reactions in normal guinea pigs and inactivated C'. On a weight basis, the soluble complexes and nonspecifically aggregated {gamma}-globulin were comparable with respect to these biologic activities.

Footnotes

1 This study was supported by Research Grant E-3308 from the National Institute of Allergy and Infectious Diseases, U. S. Public Health Service.

2 Present address: Children's Asthma Research Institute and Hospital, Denver, Colorado.






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