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The Journal of Immunology, 1963, 90: 925-928.
Copyright © 1963 by The American Association of Immunologists, Inc.
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The Effect of Ionic Strength on the Formation, Stability and Lysis of Persensitized Erythrocytes (EAC'142)

Harold G. Walker1 and Alastair C. Wardlaw

From the Connaught Medical Research Laboratories, University of Toronto, Toronto 4, Canada

Abstract

This investigation has shown that at least two of the intermediate reactions in the over-all process of immune hemolysis are strongly influenced by ionic strength and proceed optimally at values in the region of 0.07 to 0.08 rather than at the physiologic ionic strength 0.155. The reactions so influenced are a) the formation of EAC'142 from EA, and b) the lysis of EAC'142 by C'3. The former reaction has a sharper optimum than the latter.

In contrast, two other reactions were found to be quite unaffected by changes in ionic strength over the wide range 0.035 to 0.3. These were a) the initial sensitization reaction in which EA is formed, and b) the decay reaction of EAC'142 to EAC'14. The ionic strength independence of the latter suggests that the foward reaction in which EAC'414 is converted to EAC'142 may also be indifferent to ionic strength. Hence the dependence on ionic strength in the formation of EAC'142 from EA probably resides in either or both of the previous steps in which EAC'1 and EAC'14 are successively formed.

These observations help to explain the previously reported finding (1) that the optimum ionic strength for the over-all process of immune hemolysis is close to 0.08.

Footnotes

1 Senior student, Faculty of Medicine, University of Toronto, Toronto, Canada.






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