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The Journal of Immunology, 1963, 90: 829-835.
Copyright © 1963 by The American Association of Immunologists, Inc.
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In Vitro Production of Antibody by Chicken Spleen Cells

I. Detection of Antibody by Isotope Techniques1

Roy Patterson, Irena M. Suszko and Jacob J. Pruzansky

From the Allergy Research Laboratory, Department of Medicine, Northwestern University Medical School, Chicago, Illinois

Abstract

Chicken spleen cells were cultured in vitro after in vivo antigenic stimulation with bovine serum albumin. The production of chicken antibody was detected by several techniques.

Quantitative estimations of antibody by an ammonium sulfate coprecipitation technique provide another method of detection of antibody in tissue culture medium.

Footnotes

1 This investigation was supported by Research Grant AI-04199 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service.






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