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Immunochemistry of Coagulated Antigen-Antibody Complex

I. General Procedure and Effects of Coagulating Conditions¹

From the Department of Physiology and Pharmacology, University of Saskatchewan, Saskatoon, Canada

Abstract

Under suitable conditions, a number of chemical agents and/or heat may be used to render insoluble either antibody molecules or specific complexes, without destroying or masking all of the native combining sites. These coagula may be used as highly specific adsorbents for native antigen and the antigen may be subsequently eluted at pH 3.0. A study of some suitable coagulating reagents and conditions has been presented.

Footnotes

¹ This investigation was supported in part by Public Health Service Research Grant No. H-5795 from the National Heart Institute, USPHS, and in part by the U.S. Air Force School of Aerospace Medicine, Aerospace Medical Division (AFSC) under Contract No. AF 41 (657)-320.