HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yokoyama, M. Articles by Brady, R. O. Search for Related Content PubMed Articles by Yokoyama, M. Articles by Brady, R. O.

Immunochemical Studies with Gangliosides

From the Laboratory of Neurochemistry, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland

Abstract

The injection of purified gangliosides caused the production of specific antibodies in rabbits when administered with a carrier substance such as rabbit or sheep erythrocytes, Freund's adjuvant, whole human serum or bovine serum albumin. A separate antibody was also produced by the injection of erythrocytes coated with asialogangliosides. Anti-ganglioside and anti-asialoganglioside antibodies were demonstrated by hemagglutination, complement fixation and passive cutaneous anaphylaxis tests.

Gangliosides exhibited blood group M and Rho(D) activity in hemagglutination inhibition tests and some, although weaker, blood group substance A and N activity. The intact ganglioside molecule was required for blood group activity. Asialogangliosides and crystalline N-acetylneuraminic acid were inactive in this regard.

Footnotes

¹ Present address: Department of Medicine, University of California, San Francisco Medical Center, San Francisco, California.