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Quantitative Studies of a Conglutinating System in Normal Bovine Serum

I. Isolation of Conglutinin, Antibody and Components of Complement¹

From the Department of Surgical Research, St. Luke's Hospital, Cleveland, Ohio

Abstract

A conglutinating system present in normal bovine serum has been shown to consist of antipolysaccharide, conglutinin and complement. Conglutinated dextran yielded conglutinin, C'1 and C'4 on extraction with EDTA, and both high and low molecular weight antidextran on extraction with maltose. Details of the chromatographic purification of conglutinin and the high and low molecular weight antidextran are given. The isolated conglutinin was essentially homogeneous by free electrophoresis and by ultracentrifugation, but was heterogeneous immunochemically. Conglutinin migrated as a -globulin, had a sedimentation constant of 6.6 and a molecular weight of 650,000.

Footnotes

¹ Supported by Grant E2312 from the National Institutes of Health.

This article has been cited by other articles:

				M. A. Leon and R. Yokohari Conglutination: Specific Inhibition by Carbohydrates Science, March 20, 1964; 143(3612): 1327 - 1328. [Abstract] [PDF]