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Preparation and Immunologic Comparison of Antibodies to Closely Related Antigens Produced in Individual Rabbits¹

From the Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, N. Y.

Abstract

A procedure for the isolation of two antibodies specific for very similar antigens, glucosyl-phenylazo bovine serum albumin and galactosyl-phenylazo bovine serum albumin, from doubly-immunized individual rabbits is described. Immunoelectrophoretic and ultracentrifugal analyses of these purified antibodies failed to reveal differences between them, indicating only one 7 S component for each; while total amino acid analyses and total sugar analyses also failed to reveal differences in composition. Factors which may be responsible for their difference in specificity are discussed.

Footnotes

¹ Supported by Public Health Service Grant E-1763 and National Science Foundation Grant G-14420.

² Postdoctoral Research Fellow of the National Institute of Dental Research, USPHS. Present address: National Institute of Dental Research, USPHS, Bethesda, Maryland.