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Phenotypic Expression of Isoantigens of the H-2 System in Embryonic and Newborn Mice¹

From the Department of Tumor Biology, Karolinska Institute Medical School, Stockholm 60, Sweden

Abstract

Previous studies indicated that red blood cells and spleen cells of newborn A, C3H and CBA mice were resistant to humoral H-2 antibodies during the first 3 days after birth. It was demonstrated in the present work that this difference in timing with regard to the phenotypic expression of the various H-2 components was preserved when they were brought together on the same F₁ hybrid erythrocytes or spleen cells.

It was demonstrated by the indirect fluorescent antibody technique that H-2 antigens were present on all spleen cells of newborn mice, but the frequency of positively stained cells diminished rapidly with decreasing age in embryos and no antigenic cells could be detected in 15-day-old embryos.

X-irradiated cells of 13- to 14-day-old embryos were found to be antigenic, as judged by their capacity to induce transplantation immunity against skin grafts and by their ability to stimulate the formation of humoral antibodies.

It is suggested that the difference with regard to the time of phenotypic expression of H-2 isoantigens as shown by the various techniques is due to differences in the relative sensitivity of the methods used rather than to differences in the kind of isoantigens detected by the different techniques.

Footnotes

¹ This work was supported by grants from the Swedish Cancer Society, and by Grant C-3700 from the National Cancer Institute, United States Public Health Service, and by a grant from the Swedish Medical Research Council. The author wishes to thank Professor G. Klein and Dr. E. Klein for their valuable help and great interest during the investigation.