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Antigenic Analysis of Influenza Viruses by Complement Fixation

VII. Further Studies on Production of Pure Anti-S Serum and on Specificity of Type A S Antigens¹

From the Division of Virology, The Department of Public Health and Preventive Medicine, School of Medicine, University of Pennsylvania, the Virus Diagnostic Laboratory, Reference Laboratory of the Department of Health, Commonwealth of Pennsylvania, and The Children's Hospital of Philadelphia, Pennsylvania

Abstract

An improved method is described for producing in guinea pigs sera which contain antibodies only to the soluble (S) antigens of type A influenza viruses and none to the viral (V) antigens. The critical modification was the introduction of S antigen derived from A/equi to serve as the booster injection to recall anti-S which was initially stimulated by active Swine/Cam virus. Since A/equi shares no V antigen relationship with either swine or human influenza strains, concomitant recall of anti-V in animals in which this antibody had already disappeared, by any undetectable V components remaining in the S antigen, was avoided.

The anti-S sera thus produced reacted the same in the complement fixation test, whether titrated against S antigens derived from A/equi, S/Cam or three prototype human strains. Similarly, no strain-specific differences could be demonstrated among the S antigens prepared from these five type A viruses.

Footnotes

¹ The work described in this paper has been supported by a grant-in-aid from the National Institutes of Health, U. S. Public Health Service.