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Immunochemical Study of Rabbit -Globulin Allotypes¹

From the Department of Bacteriology and Immunology, Harvard Medical School, and the Department of Medicine, Massachusetts General Hospital, Boston

Abstract

Antibodies to -globulin allotypes were produced in rabbits by immunization with purified rabbit -globulin. These antibodies were studied quantitatively by precipitin reaction and shown to be directed towards antigenic determinants occurring on the antibody combining Fragments I and II obtained by papain digestion of rabbit -globulin.

Similar allotype specific antibodies could be produced in goats by immunization with one type of rabbit -globulin and absorption with another type. One such goat antiserum was found to be capable of precipitating with whole rabbit -globulin of the appropriate allotype but not with the papain digest fragments obtained therefrom. Such fragments were, however, shown to inhibit precipitation with whole rabbit -globulin, thus indicating their apparent univalence.

When rabbit -globulin in the form of a specific precipitate in Freund adjuvant was used for immunization, an antibody specific for human -globulin was found in addition to the rabbit allotype specific antibody.

Footnotes

¹ Supported by Grant E-1289 from the U. S. Public Health Service.