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The in Vitro Detection of "Cell-Fixed" Hemagglutinating Antibodies to Tuberculin Purified Protein Derivative (PPD) in Humans¹

From the McGill University Clinic of the Montreal General Hospital and the Departments of Biochemistry and Chemistry, McGill University

Abstract

1. Extracts of the washed peripheral blood leukocytes of all adult human subjects studied were found to contain hemagglutinating antibodies to tuberculin purified protein derivative (PPD). Subjects with positive tuberculin skin tests had significantly higher titers of these "cell-fixed" hemagglutinating antibodies to tuberculin PPD than did tuberculin skin test negative subjects.

2. The finding of low titers of "cell-fixed" antibodies in tuberculin skin test negative subjects may reflect minor degrees of tuberculin hypersensitivity not detected by skin testing, or may be due to cross-reactivity with Mycobacteria other than Mycobacterium tuberculosis.

3. The "cell-fixed" antibodies are probably adsorbed from the serum of the leukocyte donor.

4. No hemagglutinating antibodies to tuberculin PPD were found in sera or leukocytes obtained from the umbilical cord blood of newborn infants.

5. The relationship between "cell-fixed" hemagglutinating antibodies to tuberculin PPD and "transfer factor" was not established in this investigation.

Footnotes

¹ This work was supported by research grants from the Medical Research Council of Canada, Ottawa, Ontario, and from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. The results were presented in part before the 18th Annual Meeting of the American Academy of Allergy, February 6, 1962.

² Medical Research Fellow, Medical Research Council of Canada.

³ This work was performed during the tenure of a summer scholarship from the Allergy Foundation of America.

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