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Photometric Methods for the Measurement of Hemagglutinating Viruses and Antibody

I. Further Experience with a Novel Photometric Method for Measuring Hemagglutinins¹

From the Department of Epidemiology and Virus Laboratory, School of Public Health, University of Michigan, Ann Arbor, Michigan, and Robert Koch-Institut, Berlin N65, Germany

Abstract

A photometric method for titration of virus using equipment available in this country has been described. The standard deviation of mean titer values found in a series of replicate titrations carried out in one laboratory was 3.5%. The average difference in titer values found in three different laboratories using several strains of virus was 7.87%. Such reproducibility has not previously been achieved and the use of the method may permit development of an international standard for measurement of hemagglutinin.

Footnotes

¹ This investigation was conducted under the auspices of the Commission on Influenza, Armed Forces Epidemiological Board, and was supported by the Office of the Surgeon General, U.S. Army, Washington, D.C.