HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Feigen, G. A. Articles by Terres, G. Search for Related Content PubMed PubMed Citation Articles by Feigen, G. A. Articles by Terres, G. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Dietary Proteins

Effect of Antibody Concentration and Temperature upon Physical Adsorption and Histamine Release¹

From the Department of Physiology, Stanford University, Stanford, California

Abstract

An investigation was made into the nature of passive in vitro sensitization of guinea pig ileum by parallel studies of the effect of temperature on the physical adsorption of antiovalbumin and the magnitude of histamine release following challenge with antigen.

1. The uptake of radioiodinated rabbit -globulin by strips of guinea pig ileum was studied for several concentrations of protein at temperatures ranging from 20° to 37°C. There was no essential difference in the rates of physical adsorption among the several categories tested, the average first order velocity constants being 0.020 and 0.024 min^-1 for 20° and 37°C, respectively.

2. Tissues immersed for 60 min in concentrations of antiovalbumin ranging from 0.046 to 0.273 mg/ml at 20°C and 27°C, and from 0.009 to 0.228 mg/ml at 37°C, took up labeled antibody according to a simple function of the bulk phase concentration. The relationship between the bulk phase concentration and the amount physically adsorbed was not affected by temperature.

3. Antibody that had been adsorbed to tissue during the first incubation phase could be eluted by washing with Tyrode's solutions, but the amount eluted was not influenced by temperature. When these tissues were subsequently challenged in the presence of 0.1 mg/ml ovalbumin a constant proportion of label was removed but, as control experiments showed, this was no greater than that which could be achieved by washing with Tyrode's.

4. The release of histamine from tissues of actively immunized animals was increased by elevating the temperature during the challenge step. Variations of temperature during the adsorption step that preceded challenge had no effect upon histamine release if the subsequent challenge temperature was constant.

5. Although the amount of antibody physically adsorbed was influenced only by bulk phase concentration and not by temperature, the quantity of histamine released for a given degree of physical adsorption was greatly increased as the temperature of the adsorption step was elevated, the activation energy of this process being 18 kcal.

Since both physical adsorption and histamine release depend upon the concentration of antibody in the bulk phase, the relationship between these two processes is not a specious one. It is our view that the effect of temperature observed in these studies is to activate receptor sites in such a way as to increase the proportion of histamine-bearing cells which are in a critical state preceding challenge with antigen.

Footnotes

¹ Supported by Grant USPHS H-3693 and by Contract 225 (46) between Stanford University and the Office of Naval Research.

² Senior Research Fellow, U. S. Public Health Service (GSF-362-C1).

This article has been cited by other articles:

				G. A. Feigen and C. B. Nielsen Passive Sensitization in vitro: Effect of Antibody Concentration on the Lag Period and Velocity Science, November 4, 1966; 154(3749): 676 - 677. [Abstract] [PDF]