HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Engle, C. G. Articles by Liu, O. C. Search for Related Content PubMed Articles by Engle, C. G. Articles by Liu, O. C.

Studies on the Chemotherapy of Viral Infections

III. Anti-Influenza Activities of Glyoxal Analogs in Vitro and in Chick Embryo Systems

From the Research and Development Division, Smith Kline and French Laboratories, Philadelphia, Pennsylvania

Abstract

Three compounds containing the ketoaldehyde moiety were studied for activity against the PR8 strain of influenza A virus in vitro and in various embryonated egg systems. These compounds were p - (p - phenylphenacylideneamino)benzoic acid (8949); 4,4'-biphenyldiglyoxylaldehyde, dihydrate (8234); and p-(-ethoxy-p-phenylphenacylamino)benzoic acid (8318). All were found to be virucidal against the virus in vitro. When these compounds were suspended in 25% heat-inactivated calf serum, the virucidal effect was diminished by approximately 30- to 100-fold. Also, the virucidal effect was reduced by mixing compound with allantoic fluids in vitro or directly in ovo. All compounds were shown to be effective against the PR8 virus in ovo when the compounds and the challenge virus were given by the allantoic route. However, suspending 8234 in 25% calf serum did not impair its activity in intact eggs. Both 8234 and 8318 were studied in de-embryonated eggs using the one-step growth curve technique. A suppression of the hemagglutinins present in the fluids as well as that contained in the allantoic membrane was observed in the treated as compared with the untreated controls when initial treatment with these compounds was begun as early as 0 to 6 hr or as late as 11 to 12 hr postinfection. Thus, these compounds were shown to have both a virucidal and virustatic effect.

Footnotes

¹ Present address: The National Drug Company Research Laboratories, Philadelphia 44, Pennsylvania.