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Formation of Incomplete Influenza Virus in Ascites Tumor Cells

Studies on Complement-Fixing "S" Antigen¹

From the Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

Abstract

The formation of noninfectious, cell-associated viral hemagglutinins in Krebs 2 ascites tumor cells inoculated with large amounts of influenza virus, PR₈ strain, was accompanied by high titered complement-fixing soluble antigen. This antigen appeared 1 to 2 hr before the hemagglutinin, was less affected by adverse conditions, and could be found in cells with a dilute input of virus which was insufficient for hemagglutinin formation.

Comparison to other influenza systems in deembryonated eggs and HeLa cells revealed no significant quantitative differences in the production of the intracellular hemagglutinin and S antigen, irrespective of whether complete or incomplete virus was being formed.

The formation of hemagglutinin could be preferentially inhibited by dilution of the virus input, by 0.1% glutamine, by low concentration (0.005 to 0.01%) of (NH₄)₂HPO₄, or by a 15-min delay in addition of higher concentrations of ammonium salts.

Proflavine had no effect in this system.

Footnotes

¹ These studies were supported by Research Grant C-3762 from the U. S. Public Health Service and by funds from the Eugene Higgins Trust.