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The J Substance of Cattle

VII. Production of Immune Anti-J in Rabbits¹

From the Departments of Biochemistry and Genetics, University of Wisconsin, Madison, Wisconsin

Abstract

A heat-stable fraction from cattle serum containing J substance produced strong and specific antisera for J in 9 of 17 rabbits. Preparations from urine, abomasal mucosa and untreated serum showing high J activity were at best weakly antigenic. The difference in antigenicity of these preparations is not explained. The titers and avidities of the immune anti-J sera were as high as, but not generally higher than, those of normal anti-J sera.

Footnotes

¹ Paper No. 862, Division of Genetics. Published with the approval of the Director of the Agricultural Experiment Station, University of Wisconsin. This project was supported in part by the Research Committee of the Graduate School from funds supplied by the Wisconsin Alumni Research Foundation and in part by Research Grant E-1643 from the Department of Health, Education and Welfare, of the National Institutes of Health, Public Health Service.