HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ehrlich, G. Articles by Manski, W. Search for Related Content PubMed PubMed Citation Articles by Ehrlich, G. Articles by Manski, W.

Cytotoxicity of Anti-Tissue Antibodies

II. Effects of Anti-Lens Sera in Tissue Culture¹

From the Department of Ophthalmology Research, Columbia University, College of Physicians and Surgeons, and the Institute of Ophthalmology, Columbia-Presbyterian Medical Center, New York

Abstract

1. Duck anti-adult rabbit lens sera demonstrated striking cytotoxicity against rabbit lens epithelium in tissue culture, whereas unimmunized duck serum was without effect. In addition, duck antisera to 26-day-old fetal rabbit lenses revealed similar cytotoxic effects.

2. Duck antisera to rabbit sclera or cornea showed only minimal damaging effects on rabbit lens epithelium. These data confirm and extend earlier observations on the differences in specificity of cytotoxic antigens in various tissues from a single species.

3. Homologous antisera prepared in the rabbit against pooled rabbit lenses, did not reveal cytotoxic effects on rabbit lens epithelium.

4. Rabbit antisera against human, bovine, frog and marine fish (menhaden) similarly failed to damage rabbit lens epithelial cells, although all of the antisera contained numerous precipitating organ-specific antibodies active against rabbit soluble lens components. The data thus indicate that the antigens involved in organ specificity of the lens were not concerned in the cytotoxic reactions.

Footnotes

¹ These studies were supported by a research grant from the United States Public Health Service.