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Characteristics of Endotoxin Altering Fractions Derived from Normal Human Serum¹

From the Department of Bacteriology, The University of Michigan, Ann Arbor

Abstract

Alcoholic fractionation of normal human defibrinated plasma by Cohn's method 10 resulted in two fractions capable of altering bacterial endotoxins. Incubation of endotoxin in the first, Fraction IV₁, resulted in a marked reduction of the ability of specific antiserum to precipitate endotoxin. In addition, the capacity of the lipopolysaccharide to elicit a pyrogenic response in rabbits was abolished. A second fraction, III₀, as isolated, was generally incapable of such effects unless it was activated by several procedures. All other fractions did not affect these properties of endotoxin. Alteration of the lethal activity of endotoxin was not evident after incubation in Fraction IV₁, but was reduced after exposure to activated Fraction III₀. The characteristics of these reactions are recorded.

Footnotes

¹ This investigation was supported by grants from the U. S. Public Health service (E 1524) and The Upjohn Company, Kalamazoo, Michigan. Presented in part before the American Society of Microbiology, May 1960.

² F. G. Novy Fellow in Department of Bacteriology. Present address: Dept. of Bacteriology, Keio Gijuku University, Tokyo, Japan.