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Electron Microscopic Counting of Virus Particles by Sedimentation on Aluminized Grids¹

From the Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas

Abstract

The technique described employs collodion covered grids which have been coated with a thin layer of aluminum. Negatively charged virus particles are sedimented at high speed directly upon the positively charged aluminized grid surface. Grids may be washed under a stream of water to remove soluble salts and further purify poliovirus without detectable loss of virus particles. Particle distribution is satisfactory for counting with the electron microscope. Precision of counting is quite high when substantial numbers of particles are counted, and the number of particles counted per unit area is directly proportional to the relative concentration. The relative simplicity of the method permits numerous determinations to be made within a short period of time. The method has been used satisfactorily with poliovirus, bacteriophage and vaccinia virus, three viruses differing greatly in size and morphology.

Footnotes

¹ Aided by a grant from The National Foundation, by a Training Grant 2E-74 from the National Institute for Allergy and Infectious Diseases, and by Research Grant C-4600 from The National Cancer Institute.