HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Allerhand, J. Articles by Zitrin, C. M. Search for Related Content PubMed PubMed Citation Articles by Allerhand, J. Articles by Zitrin, C. M.

Detection of Antibodies in the -2 and -Globulin Fractions of Human Tuberculous Sera

From the Department of Pediatrics, Long Island Jewish Hospital, New Hyde Park, New York

Abstract

Human tuberculous and nontuberculous sera were fractionated by means of the continuous flow paper electrophoresis method. Precipitin tests were then performed, using the -2 and -globulin fractions as the sources of antibody, with OT, PPD, and an unheated culture filtrate of tubercle bacilli as the sources of antigen. Both the tuberculous and control sera gave negative results before fractionation. After fractionation, the control sera remained negative. Of the 14 patients' sera, 12 became positive, 7 with both fractions, 3 with -globulin alone and 2 with -2 globulin alone. The -2 globulin fraction, in those cases where both were positive, generally precipitated earlier than the -globulin fraction.

In a comparison between the agar doublediffusion test with whole serum and the precipitin test with fractions of the same serum, the latter was positive in seven out of eight samples, whereas the former was positive in three out of the eight.

Footnotes

¹ This study was supported by Research Grant E-2482 from the Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service.