HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tokumaru, T. Search for Related Content PubMed PubMed Citation Articles by Tokumaru, T.

A Kinetic Study on the Labeling of Serum Globulin with Fluorescein Isothiocyanate by Means of the Gel Filtration Technique¹

From the Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire

Abstract

1. It was found that Sephadex gel filtration could be used for the quantitative analysis of reactants by rapid separation.

2. Using simultaneous gel filtration of samples in a number of sister columns association constants and the number of sites were obtained for the kinetics of the labeling process of globulins with fluorescein.

3. Reagents for protein precipitation were used to remove superficially adsorbed dye from fluorescent atibody. The results of such precipitation showed that the gel-filtered material contained smaller amounts of adsorbed dye than dialyzed material.

4. The patterns of DEAE-cellulose and DEAE-Sephadex column chromatography were compared by the use of fluorescent antisera.

5. It was shown that the complex of papain and fluorescent antibody could be separated from free papain satisfactorily on a Sephadex G-75 column.

Footnotes

¹ This work was supported by Grant B-2685(C1) from The National Institutes of Health, Bethesda, Maryland.

² Present address: The Children's Hospital of Philadelphia, Department of Pediatrics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.