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Interferon and Viral Ribonucleic Acid

Effect on Virus-Susceptible and Insusceptible Cells¹

From the Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota

Abstract

The action of interferon in inhibiting either visible virus effect on cells or viral multiplication is intracellular. The evidence suggests that very soon after its adsorption interferon acts to delay virus synthesis in cells naturally susceptible to virus as well as those naturally insusceptible, even when interferon is added after viral ribonucleic acid (RNA) adsorption to cells is completed. This action occurs beyond the point of cellular adsorption, penetration, and removal of virus protein without gross effect upon virus release. It further appears that interferon does not block intracellular sites of attachment for viral multiplication, nor does its effect depend upon direct inactivation of parental viral RNA.

Interferons produced in the allantoic sac of chicken embryos by a myxovirus (influenza A) or an arthropod-borne virus (Japanese encephalitis) had similar inhibitory effects on chicken embryo cells against Western equine encephalitis virus as well as against the RNA extracted from poliovirus Types I and II. Avian interferon had no detectable effect on susceptibility of HeLa cells to poliovirus RNA.

Footnotes

¹ Presented in part at the Annual Meeting of the American Association of Immunologists, Atlantic City, New Jersey, April 12, 1961. Supported by grants from the National Foundation and the Armed Forces Epidemiological Board Commission on Viral Infections to Dr. J. T. Syverton and Dr. W. F. Scherer, respectively.

² This investigation was carried out during the tenure of a Postdoctoral Fellowship from the National Institute of Allergy and Infectious Disease and a medical career development award.

³ Present address: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington.

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				K. Paucker Basic Science Review : Interferon: A Cellular Product which Inhibits Viruses Clinical Pediatrics, November 1, 1964; 3(11): 671 - 678. [PDF]