HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Grabar, P. Articles by Wostmann, B. S. Search for Related Content PubMed PubMed Citation Articles by Grabar, P. Articles by Wostmann, B. S.

Immunoelectrophoretic Analysis of the Serum of Germfree Rats¹

From the Service de Chimie Microbienne, Institut Pasteur, Paris, France; and the Lobund Laboratories, University of Notre Dame, Notre Dame, Indiana, U.S.A.

Abstract

By using immunoelectrophoretic techniques and anti-germfree rat and anti-conventional rat immune rabbit sera it was shown that the serum of germfree rats contains the same major antigenic components observed in the serum of conventional rats. Certain constituents, especially those of low mobility, are present in such small amounts that detection is difficult, even with methods as sensitive as specific precipitation. Only small amounts of -globulin were detectable by direct analysis. These "germfree" -globulins showed a relatively high mobility. Injection of germfree rat serum into rabbit produced the entire spectrum of "anti-" antibody otherwise found upon immunization with conventional rat serum.

Footnotes

¹ At the Lobund Laboratories this research was supported specifically by the National Science Foundation (Grant G-6120); also generally by the Office of Naval Research and the Army Surgeon General (Contract NONR-1623), by the National Institutes of Health (Grant A-566), and by the University of Notre Dame.