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Immunological Studies on Rat Parabiosis¹

From the Department of Bacteriology and Immunology, University of Buffalo School of Medicine, Buffalo, N. Y.

Abstract

Parabiosis was established between unrelated female albino rats weighing 140 to 200 g, according to the technique of Bunster and Meyer. All serological tests performed in vitro for the detection of antibodies against antigens present in rat erythrocytes, leukocytes, serum, and various organs were negative, or at least not conclusive.

In contrast, sera of 37 out of 91 parabiotic rats induced skin reactions when injected intradermally into normal test rats. The skin reactions became visible after intravenous administration of Evans blue.

The skin reaction producing factor (SRPF) was to be found in the serum as early as 5 to 15 days after parabiotic union. The individuality of the skin-tested animal was of primary importance in obtaining positive reactions, necessitating the examination of more than one test animal for each serum under investigation.

The parabiotic animals sometimes, but not always, exchanged their SRPF. The passively transferred SRPF originating from the partner's serum disappeared within 2 to 3 weeks after the separation of the animals, whereas the actively produced SRPF persisted up to 8 weeks.

On the basis of the experimental data presented, the SRPF was considered an isoantibody directed against group-specific tissue antigens of the parabiotic partner.

Footnotes

This work was supported in part by a grant from the National Foundation, and in part by Research Grant C-2357 from the National Cancer Institute, National Institutes of Health, United States Public Health Service.