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Aspects of the Immune Hemolytic Reaction

VI. Effect of Inhibitors from Sheep Serum on Immune Hemolysis¹

From the Division of Laboratories and Research, New York State Department of Health, Albany, the College of Physicians and Surgeons, Columbia University, New York, and the Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey

Abstract

Sheep serum contains inhibitors that prevent the lysis of sensitized sheep red cells by guinea pig, pig or human C', but do not interfere with the sensitization of the cells by rabbit antibody. The presence of a relatively large proportion of sheep serum during the formation of EAC'_1,4,2 by guinea pig C' results in slight loss of C'1 and C'2 activities on these cells and no detectable change in C'4 activity. The inhibitors affect primarily the C'3 step. Sheep serum, however, lyses sensitized human erythrocytes, and human serum inhibits the lysis of type A human erythrocytes by sheep C'. These results suggest a reason why a given C' may be less active in lysing sensitized homologous red blood cells.

Footnotes

¹ This work was taken in part from the Ph.D. dissertation presented by one of us (K. A.) to the Faculty of Pure Science, Columbia University. A preliminary report was given before the Federation of American Societies for Experimental Biology in April, 1957, at Chicago, Illinois. We should like to thank the National Science Foundation and the Office of the Surgeon General, Department of the Army, for support of the work in part.