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Children's Hospital of Philadelphia and the Department of Pediatrics, University of Pennsylvania, Philadelphia, Pennsylvania
Abstract
Young rabbits were used as donors of lymph node cells for incubation in vitro with Shigella-trypsin filtrate and transfer to irradiated recipient rabbits. No agglutinins to Shigella could be detected subsequently in the sera of the recipient animals if the donors were less than 1 month of age. Thereafter, a progressive rise in maximal antibody level of recipients was found with increasing age of the donors, up to an age of approximately 3 months. Donor rabbits injected with Shigella in the 1st month of life yielded cells which, again, were ineffective. Thereafter, the effectiveness of the cells increased substantially more rapidly with increasing age of donor than in the case of antigen-incubated cells.
On active "immunization" of young rabbits with Shigella-trypsin filtrate at an adequate dosage level, agglutinins were detected only after the 1st month of life. With larger doses of this antigen, the antibody appeared in younger rabbits, but at a later time relative to the injection of antigen and at a relatively low titer. For comparison, other young rabbits were injected with alum-precipitated bovine 
-globulin. Anti-BGG could be detected in the sera of some rabbits injected as early as 3 days of age, and regularly in rabbits injected after the 1st week of life.
Footnotes
* This study was supported by Research Grant H-4598 of the National Heart Institute, National Institutes of Health, U. S. Public Health Service.
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