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Endotoxin and Its Relationship to Anaphylaxis in the Mouse

Departments of Dermatology, Microbiology, and Surgery, Columbia University, College of Physicians and Surgeons, and First Surgical Division, Bellevue Hospital, New York

Abstract

Endotoxins isolated from Gram-negative organisms have been shown to modify the response of experimental animals to various forms of injury such as thermal burns (1), hemorrhage (2), tourniquet (3), whole body irradiation (4), anaphylaxis (5), or massive bacterial (6) or viral infections (7). Depending on circumstances, the endotoxins may enhance the mortality or offer protection from the fatal outcome of the injury. This report describes some effects of an endotoxin on anaphylaxis in mice.

Materials and Methods. The endotoxin used was the lipopolysaccharide of Escherichia coli purchased from the Difco Company. White, Swiss, female mice weighing 18 to 20 g were sensitized by intraperitoneal (i.p.) injections of normal horse serum every other day for a series of four injections, and were challenged intravenously 21 days after the first sensitizing injection, as described previously (8). Tolerance to endotoxin was produced by a series of five daily injections starting with 25 mµ of endotoxin and then increasing each injection by 25 mµ.