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The Journal of Immunology, 1962, 88: 109-112.
Copyright © 1962 by The American Association of Immunologists, Inc.
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Demonstration of Brucella Antibodies in Human Serum

A Comparison of the Fluorescent Antibody and Agglutination Techniques

Joseph Z. Biegeleisen, Jr., Betsy R. Bradshaw and Max D. Moody

United States Department of Health, Education and Welfare, Public Health Service, Communicable Disease Center, Atlanta, Georgia

Abstract

Brucella antibody titers were determined on 96 human serum specimens by the conventional tube agglutination and indirect fluorescent antibody tests. The agglutinin titers were routinely higher than those obtained by the fluorescence technique, but fluorescence always was observed when the serums contained antibodies demonstrable by agglutination tests. Comparisons were made of the titers obtained by both methods and their significance was discussed.






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