HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhdanov, V. M. Articles by Azadova, N. B. Search for Related Content PubMed PubMed Citation Articles by Zhdanov, V. M. Articles by Azadova, N. B.

Fluorescent Microscopic Study of Sendai Incomplete Virus Production in Tissue Culture Cells

Institute of Virology, Moscow, Russia

Abstract

When monkey kidney cells were infected with 1 EID₅₀ of Sendai virus per cell, infectious virus was produced and the cytoplasmic inclusions could be observed by the fluorescent antibody indirect method. Antigen was never seen in the nuclei.

Diffuse fluorescence of the cytoplasm without cytoplasmic inclusions could be seen after inoculation with 100 EID₅₀ per cell. Here, presumably incomplete virue production with a low EID₅₀:HA ratio had taken place.

Incomplete Sendai virus was also formed in monkey kidney tissue culture in the presence of proflavine. The cytopathic effect appeared earlier and was more intense than in controls. The fluorescent antibody indirect technique detected the cells as large, structureless brightly fluorescing globi with a high concentration of antigen in the cytoplasm as well as in the nuclei. Acridine-orange staining showed profound changes in the nucleus and the lack of RNA in newly-formed antigen. The possible role of the nucleus in incomplete virus production is supposed.