HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ishizaka, T. Articles by Borsos, T. Search for Related Content PubMed Articles by Ishizaka, T. Articles by Borsos, T.

Biological Activity of Aggregated -Globulin

IV. Mechanism of Complement Fixation¹

From the Departments of Microbiology and Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland

Abstract

1. Aggregated human -globulin, obtained by heating, inactivated C'₁ and/or C'₄, as well as C'₂ and C'₃.

2. With respect to the successive steps of the complement inactivation, the aggregated -globulin fixed C'_1,4, followed by inactivation of C'₂.

3. Inactivation of C'₃ in whole complement by the aggregated -globulin was inhibited in the presence of EDTA, due to blocking of the preceding steps.

4. The similarity in the mechanism of complement fixation by antigen-antibody systems and by aggregated -globulin was discussed.

Footnotes

¹ This is the third of a series of studies carried out in the laboratory of Dr. Abraham G. Osler under the auspices of the Commission on Cutaneous Diseases of the Armed Forces Epidemiological Board, and supported in part by the Office of the Surgeon General, Department of the Army, and the National Science Foundation. We also wish to thank Drs. Manfred M. Mayer and Herbert J. Rapp for helpful advice and criticism.

² Present Address: The National Institute of Health, Tokyo, Japan.

³ Post-doctoral Research Fellow of the National Heart Institute, NIH, United States Public Health Service, 1958 to 1960.