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Mouse Antibody

I. Characterization and Properties of Antibody in Mouse Peritoneal Fluid¹

From the Stella Duncan Memorial Laboratory and Department of Microbiology, Montana State University, Missoula, Montana

Abstract

It has been shown that peritoneal fluid of mice immunized intraperitoneally with Ea in Freund's adjuvants may contain as much as 2 mg of Ab N/ml. Qualitatively, mouse peritoneal fluid appears to be very similar to mouse serum. Mouse antibody behaves like rabbit antibody in the quantitative precipitin test; insoluble precipitates are formed in extreme antibody excess, and soluble complexes are formed in extreme antigen excess. The anti-Ea antibody, the only one studied, was found only in the -globulin portion of the fluid. Various factors affecting the precipitin reaction such as incubation temperature, length of incubation and NaCl concentration have been investigated, and conditions which permit satisfactory and reproducible results have been established.

Footnotes

This work was supported in part by Grant E-1832 from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health, United States Public Health Service.

² Present address: Rocky Mountain Laboratory, Hamilton, Montana.

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