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Quantitative Hemagglutination Inhibition Studies of Blood Group Substances

I. Assay of the Blood Group A Activity of Substances

From the Department of Immunohematology, Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, District of Columbia

Abstract

A quantitative hemagglutination inhibition technique for the precise determination of the relative effectiveness of the inhibitory strength of substances possessing blood group A activity has been developed. The method was shown to be highly reproducible and valid for blood group A substances from a variety of sources. A reversible equilibrium existing among the components of the system comprised of A substance, group A₁ cells and human anti-hog A serum was demonstrated.