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Staining of Vaccinia-Infected L Cells with Brilliant Cresyl Blue¹

Department of Bacteriology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina

Abstract

Although over 95% of normal L cells in cultures 3 to 5 days old are stained by brilliant cresyl blue, cells infected with vaccinia virus tend to remain unstained. This tendency may develop 2 hr after infection and increase to involve most of the cells.

Footnotes

This work was supported by Grant RG5177 from the Division of Research Grants, U. S. Public Health Service.

² Rockefeller Foundation Fellow. Instytut Weterynarii W Pulawach, Poland.

³ Postdoctoral Trainee on Training Grant 2E-45 from the United States Public Health Service. Present address: Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas.