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The Journal of Immunology, 1961, 86: 675-680.
Copyright © 1961 by The American Association of Immunologists, Inc.
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Complement Fixation and Ionic Strength1

Lawrence Levine, Eleanor Wasserman and Stanley Mills2

From the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts

Abstract

Experiments are described which measure the effect of ionic strength on C' fixation. It has been demonstrated that C' fixation by both protein and polysaccharide immune systems is enhanced by NaCl concentrations less than and inhibited by NaCl concentrations greater than 0.15 M. This enhancement or inhibition of C' fixation by changes in NaCl concentrations appears to affect a reaction step subsequent to antigen-antibody aggregation and prior to C'3 participation.

Footnotes

1 Aided by the National Institutes of Health (Grant E-1940). Publication No. 89 of the Graduate Department of Biochemistry, Brandeis University.

2 Investigator of the Howard Hughes Medical Research Institute.






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