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Effect of Humoral Factors on in Vitro Phagocytic and Cytopeptic Activities of Normal and "Immune" Phagocytes¹

From the Department of Bacteriology, College of Medicine, University of Utah, Salt Lake City, Utah

Abstract

Candida guilliermondi cells labeled with P³² were used in an in vitro system for quantitating phagocytosis and cytopepsis rates by mouse peritoneal phagocytes. In this system the optimal concentration of guinea pig serum was determined to be 12.5% (66 C' units/ml of buffer). Complement was not significantly inactivated at 37°C for 24 hr in serum concentrations of 25 and 12.5%, but a loss of 50% activity occurred when a serum concentration of 2.5% was employed.

Yeast phase Histoplasma capsulatum cells labeled with P³² were used in a series of experiments to study the effects of humoral factors on the ingestive and digestive activities in vitro of peritoneal phagocytes obtained from normal and immunized mice. Among humoral factors studied by this technique were complement, properdin and antibody. In all cases, a heat labile component (complement and/or properdin) not only enhanced phagocytic rates by normal and "immune" phagocytes but caused a significant enhancement of the digestive activities of the "immune" cells as compared to the normal phagocytes. Specific antibody did not appear to play any significant role in phagocytosis or cytopepsis activities of normal or "immune" phagocytes when a chronic disease agent, H. capsulatum, was used as the P³²-labeled organism.

Footnotes

¹ This work was presented in part at the 43rd annual meeting of The American Association of Immunologists, 1959 (Fed. Proc., 18: 587, 1959) and was supported by a research grant (E-2269) from the National Institute of Allergy and Infectious Diseases, Public Health Service. The material in this paper was taken from a thesis submitted by Fred Miya to the Graduate School, University of Utah, in partial fulfillment of the requirements for the degree of Doctor of Philosophy, August, 1959.

² Public Health Service Research Fellow of the National Institute of Allergy and Infectious Diseases.