HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Celada, F. Articles by Makinodan, T. Search for Related Content PubMed PubMed Citation Articles by Celada, F. Articles by Makinodan, T.

A New Model to Study Hematopoietic Transplantation Antigens

From the Biology Division, Oak Ridge National Laboratory, ² Oak Ridge, Tennessee

Abstract

A method for the study of hematopoietic transplantation antigens is described. It seems to fulfill the requirements for an immunochemical bioassay, i.e., to be simple, sensitive and quantitative. The procedure is as follows: a) Recipient mice are primed with varying doses of homologous donor antigens, and donors with sheep RBC antigen. b) Ten days later the recipients are exposed to 800 r and injected intravenously with 24 x 10⁶ spleen cells of the donor, followed by intraperitoneal injection of sheep RBC. c) Anti-sheep RBC titer is determined 6 days later and compared with the titer of nonprimed controls.

The RD₅₀ (the dose of priming donor antigen capable of stimulating the host to reject 50% of the donor spleen cells) is 6 x 10² for viable bone marrow cells, 4 x 10⁴ for bone marrow cells killed with x-rays, 1 x 10⁵ for liver cells, and 5 x 10⁵ for testicular cells. No priming potency was found with even 10⁷ RBC.

Footnotes

¹ Postdoctoral Research Fellow of the National Institutes of Health.

² Operated by the Union Carbide Corporation for the United States Atomic Energy Commission.

This article has been cited by other articles:

				B. D. Kahan and R. A. Reisfeld Transplantation Antigens Science, May 2, 1969; 164(3879): 514 - 521. [PDF]