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The Multiplication of Foot-and-Mouth Disease Virus in Trypsinized Calf Kidney and Tongue Cells and Its Use As Immunizing and Complement-Fixing Antigens

From the Plum Island Animal Disease Laboratory, Animal Disease and Parasite Research Division, Agricultural Research Service, United States Department of Agriculture, Greenport, Long Island, New York

Abstract

1. Substitution of minced calf kidney tissue for bovine tongue epithelium in suspended cultures has not proved satisfactory for propagation of foot-and-mouth disease virus. Trypsin-dispersed calf kidney cell suspension, however, supported the multiplication of the virus to significant titers.

2. The use of confluent monolayers of calf kidney cells in Povitsky flasks offers a very effective means to produce large quantities of virus for immunologic, biochemical and biophysical studies.

3. Immunogenic studies with formalin-treated tissue culture virus in mice and guinea pigs indicate that it is capable of inducing antibody titers to significant levels.

4. Acid inactivation of the virus is destructive to its immunogenic properties.

5. Foot-and-mouth disease virus multiplying on monolayers forms a complement-fixing antigen.