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Dissociation of Infective Poliomyelitis Virus from Neutralizing Antibody by Fluorocarbon¹

Division of Virology, The Department of Public Health and Preventive Medicine, School of Medicine, University of Pennsylvania and The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania

Abstract

Varying doses of poliomyelitis type I virus were mixed with appropriately diluted homologous rabbit antiserum and incubated at 37°C for 12–30 hr. The resulting noninfectious mixtures were homogenized with fluorocarbon and the amount of dissociated virus was determined by plaque assay. Similarly treated mixtures of virus and normal rabbit serum served as controls for the small losses of infectivity during incubation and homogenization. The use of optimal procedures permitted total recovery of as little as 90 to 270 plaque forming units from mixtures neutralized by an excess of antibody. Homogenization of these mixtures in absence of fluorocarbon did not yield infectious virus, indicating that the fluorocarbon is responsible for dissociation of virus from antibody.

Footnotes

This investigation was supported by Grant E-2405 of the National Institutes of Health, U. S. Public Health Service.

² Present address: Merck Institute for Therapeutic Research, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania.

³ Fellow of the Rockefeller Foundation on leave of absence from the Department of Pediatrics, Tohoku University, Medical School, Sendai, Japan.