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The Journal of Immunology, 1960, 85: 547-558.
Copyright © 1960 by The American Association of Immunologists, Inc.
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The Properdin System and Immunity

XIV. The Injection of Human Properdin Into Rabbits and the Production of Antibodies to Properdin1

Carl F. Hinz, Jr., Ralph J. Wedgwood, Earl W. Todd and Louis Pillemer2

From the Department of Medicine, Preventive Medicine, and Pediatrics and the Institute of Pathology, Western Reserve University School of Medicine, Cleveland, Ohio

Abstract

1. Partially purified human properdin was injected intravenously into rabbits. In one series of animals there was a marked rise in serum properdin level. In a subsequent series no consistent change was observed.
2. Antibodies to several constituents of human serum appeared following the initial course of injections. Antibodies to properdin did not appear after the initial injections, but did develop after restimulation.
3. The antibody to properdin interfered with the properties of human serum requiring the properdin system. These included inactivation of C'3 by Zymosan, hemolysis of PNH erythrocytes, lysis of certain bacteria, and inhibition of some viruses.
4. The antibody to properdin reacted with properdin as a single line by agar diffusion which was distinct from the other antibodies to human serum which appeared. Properdin could be distinguished from antibodies to 7S and 19S {gamma}-globulins.
5. The evidence supports the hypothesis that properdin is a single substance with multiple biologic properties.

Footnotes

1 This investigation was conducted in part under the auspices of the Commission on Immunization and Acute Respiratory Diseases, Armed Forces Epidemiological Board, and was supported by the Office of the Surgeon General, Department of the Army, Washington, D. C.; it was also supported in part by Grant H-1263C, National Heart Institute, National Institutes of Health.

2 Deceased.






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