HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pike, R. M. Articles by Schulze, M. L. Search for Related Content PubMed PubMed Citation Articles by Pike, R. M. Articles by Schulze, M. L.

Agglutination in Rheumatoid Arthritis Serum of Sheep Cells Sensitized with Hemolysin and Infectious Mononucleosis Agglutinins¹

From the Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas

Abstract

The reported failure of the sheep cell agglutination in infectious mononucleosis to be enhanced by rheumatoid arthritis serum has been confirmed. The nonreactivity of infectious mononucleosis agglutinins with rheumatoid factor was further demonstrated by the fact that sheep cells sensitized with the infectious mononucleosis antibody did not absorb rheumatoid factor. Precipitation of the agglutinins with sodium and ammonium sulfate and heating at 63°C did not alter their behavior in rheumatoid arthritis serum. Most rheumatoid arthritis sera did not enhance the agglutination of sheep cells by the serum of a guinea pig immunized with boiled beef cells. One rheumatoid serum, however, was strongly reactive in this respect.

In anion exchange cellulose (DEAE) chromatography of infectious mononucleosis serum the sheep cell agglutinins were found in fractions eluted with 0.10–0.15 M phosphate. Similar fractionation of rabbit antisheep cell serum revealed the major portion of the agglutinins in fractions eluted with 0.01 M phosphate. Some agglutinating activity was found, however, in fractions eluted with higher phosphate molarity. These latter agglutinins resembled those of infectious mononucleosis in that their activity was not enhanced by rheumatoid arthritis serum.

Footnotes

This investigation was supported in part by National Science Foundation Grant No. G-8767. Portions of this material were presented at the Annual Meeting of the American Association of Immunologists, Atlantic City, New Jersey, April 15, 1959.