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Elastase in the Exocrine Pancreas: Localization with Fluorescent Antibody¹

From the Department of Pathology, University of California School of Medicine, San Francisco, California

Abstract

The enzyme, elastase, was demonstrated in the acinar tissue of the porcine pancreas using antielastase rabbit serum and fluorescent sheep antirabbit globulin as a means of identifying and localizing the enzyme. No elastase was present in the islets of Langerhans. The specificity of the antibody to porcine elastase was demonstrated by its strong reaction with porcine pancreas and its failure to react with human or guinea pig pancreas. A positive reaction occurred between guinea pig anti-insulin serum and the islets of Langerhans of porcine pancreas whereas there was no reaction between this serum and the acinar tissue and ductal epithelium of porcine pancreas.

Footnotes

This study was supported by USPHS Grant H-1542.